Functional dissection and hierarchy of tubulin-folding cofactor homologues in fission yeast.

@article{Radcliffe1999FunctionalDA,
  title={Functional dissection and hierarchy of tubulin-folding cofactor homologues in fission yeast.},
  author={Pippa A. Radcliffe and D. B. Hirata and Leah Vardy and Takashi Toda},
  journal={Molecular biology of the cell},
  year={1999},
  volume={10 9},
  pages={
          2987-3001
        }
}
We describe the isolation of fission yeast homologues of tubulin-folding cofactors B (Alp11) and E (Alp21), which are essential for cell viability and the maintenance of microtubules. Alp11(B) contains the glycine-rich motif (the CLIP-170 domain) involved in microtubular functions, whereas, unlike mammalian cofactor E, Alp21(E) does not. Both mammalian and yeast cofactor E, however, do contain leucine-rich repeats. Immunoprecipitation analysis shows that Alp11(B) interacts with both alpha… CONTINUE READING