Functional dichotomy in the 16S rRNA (m1A1408) methyltransferase family and control of catalytic activity via a novel tryptophan mediated loop reorganization

@article{Witek2016FunctionalDI,
  title={Functional dichotomy in the 16S rRNA (m1A1408) methyltransferase family and control of catalytic activity via a novel tryptophan mediated loop reorganization},
  author={M. Witek and G. Conn},
  journal={Nucleic Acids Research},
  year={2016},
  volume={44},
  pages={342 - 353}
}
  • M. Witek, G. Conn
  • Published 2016
  • Biology, Medicine
  • Nucleic Acids Research
  • Methylation of the bacterial small ribosomal subunit (16S) rRNA on the N1 position of A1408 confers exceptionally high-level resistance to a broad spectrum of aminoglycoside antibiotics. Here, we present a detailed structural and functional analysis of the Catenulisporales acidiphilia 16S rRNA (m1A1408) methyltransferase (‘CacKam’). The apo CacKam structure closely resembles other m1A1408 methyltransferases within its conserved SAM-binding fold but the region linking core β strands 6 and 7 (the… CONTINUE READING
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