Functional coupling between glycolysis and sarcoplasmic reticulum Ca2+ transport.

  title={Functional coupling between glycolysis and sarcoplasmic reticulum Ca2+ transport.},
  author={Kai Y. Xu and Jay L. Zweier and Lewis C. Becker},
  journal={Circulation research},
  volume={77 1},
To investigate whether the energy derived from glycolysis is functionally coupled to Ca2+ active transport in sarcoplasmic reticulum (SR), we determined whether glycolytic enzymes were associated with SR membranes and whether metabolism through these enzymes was capable of supporting 45Ca transport. Sealed right-side-out SR vesicles were isolated by step sucrose gradient from rabbit skeletal and cardiac muscle. Intravesicular 45Ca transport was measured after the addition of glycolytic… CONTINUE READING
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