Functional cloning of a nucleoside diphosphate kinase from Dictyostelium discoideum.

@article{Lacombe1990FunctionalCO,
  title={Functional cloning of a nucleoside diphosphate kinase from Dictyostelium discoideum.},
  author={M L Lacombe and V Wallet and H Troll and M. Veron},
  journal={The Journal of biological chemistry},
  year={1990},
  volume={265 17},
  pages={
          10012-8
        }
}
A lambda gt11 cDNA library from Dictyostelium discoideum was screened by direct labeling of filter replicas with [35S]guanosine 5'-O-(thiotriphosphate) (GTP gamma S). A positive clone was obtained and used as probe to isolate additional clones from which a complete cDNA sequence was determined. The cDNA hybridizes to a single copy gene that is expressed as a 0.6-kilobase mRNA in vegetatively growing amoeba. The open reading frame encodes a protein of 155 amino acids (calculated Mr 16,775… CONTINUE READING
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