Functional chloride channels by mammalian cell expression of rat glycine receptor subunit.

@article{Sontheimer1989FunctionalCC,
  title={Functional chloride channels by mammalian cell expression of rat glycine receptor subunit.},
  author={Harald Sontheimer and Cord Michael Becker and Dolan B. Pritchett and Peter R. Schofield and Gabriele Grenningloh and Helmut Kettenmann and Heinrich Betz and P. H. Seeburg},
  journal={Neuron},
  year={1989},
  volume={2 5},
  pages={1491-7}
}
Cultured human cells were transfected with cloned rat glycine receptor (GlyR) 48 kd subunit cDNA. In these cells glycine elicited large chloride currents (up to 1.5 nA), which were blocked by nanomolar concentrations of strychnine. However, no corresponding high-affinity binding of [3H]strychnine was detected in membrane preparations of the transfected cells. Analysis by monoclonal antibodies specific for the 48 kd subunit revealed high expression levels of this membrane protein. After… CONTINUE READING