Functional characterization of the flagellar glycosylation locus in Campylobacter jejuni 81-176 using a focused metabolomics approach.

@article{McNally2006FunctionalCO,
  title={Functional characterization of the flagellar glycosylation locus in Campylobacter jejuni 81-176 using a focused metabolomics approach.},
  author={David J. McNally and Joseph P M Hui and Annie J Aubry and Kenneth K K Mui and Patricia Guerry and J D Brisson and Susan M Logan and Evelyn C. Soo},
  journal={The Journal of biological chemistry},
  year={2006},
  volume={281 27},
  pages={18489-98}
}
Bacterial genome sequencing has provided a wealth of genetic data. However, the definitive functional characterization of hypothetical open reading frames and novel biosynthetic genes remains challenging. This is particularly true for genes involved in protein glycosylation because the isolation of their glycan moieties is often problematic. We have developed a focused metabolomics approach to define the function of flagellin glycosylation genes in Campylobacter jejuni 81-176. A capillary… CONTINUE READING
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