Functional characterization of nuclear localization signals in yeast Sm proteins.

@article{Bordonn2000FunctionalCO,
  title={Functional characterization of nuclear localization signals in yeast Sm proteins.},
  author={R{\'e}my Bordonn{\'e}},
  journal={Molecular and cellular biology},
  year={2000},
  volume={20 21},
  pages={7943-54}
}
In mammals, nuclear localization of U-snRNP particles requires the snRNA hypermethylated cap structure and the Sm core complex. The nature of the signal located within the Sm core proteins is still unknown, both in humans and yeast. Close examination of the sequences of the yeast SmB, SmD1, and SmD3 carboxyl-terminal domains reveals the presence of basic regions that are reminiscent of nuclear localization signals (NLSs). Fluorescence microscopy studies using green fluorescent protein (GFP… CONTINUE READING