Functional characterization of highly purified human hematopoietic repopulating cells isolated according to aldehyde dehydrogenase activity.

@article{Hess2004FunctionalCO,
  title={Functional characterization of highly purified human hematopoietic repopulating cells isolated according to aldehyde dehydrogenase activity.},
  author={D. Hess and T. Meyerrose and L. Wirthlin and Timothy P. Craft and Phillip E. Herrbrich and M. Creer and J. Nolta},
  journal={Blood},
  year={2004},
  volume={104 6},
  pages={
          1648-55
        }
}
Human hematopoietic stem cells (HSCs) are commonly purified by the expression of cell surface markers such as CD34. Because cell phenotype can be altered by cell cycle progression or ex vivo culture, purification on the basis of conserved stem cell function may represent a more reliable way to isolate various stem cell populations. We have purified primitive HSCs from human umbilical cord blood (UCB) by lineage depletion (Lin(-)) followed by selection of cells with high aldehyde dehydrogenase… Expand
Selection based on CD133 and high aldehyde dehydrogenase activity isolates long-term reconstituting human hematopoietic stem cells.
TLDR
Cell selection based on ALDH activity and CD133 expression provides a novel purification of HSCs with long-term repopulating function and may be considered an alternative to CD34 cell selection for stem cell therapies. Expand
Phenotypic Characterization of Murine Primitive Hematopoietic Progenitor Cells Isolated on Basis of Aldehyde Dehydrogenase Activity
TLDR
Progenitor enrichment from Lin−cells on the basis of ALDH is a valid method whose simplicity of application makes it advantageous over conventional separations, and in vitro hematopoietic progenitor function was substantially higher in the Lin−ALDHbright population. Expand
Improved purification of hematopoietic stem cells based on their elevated aldehyde dehydrogenase activity
TLDR
Elevated ALDH activity is a broadly inclusive property of primitive human cord blood cells that, in combination with other markers, allows easy isolation of the stem cell fraction at unprecedented purities. Expand
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TLDR
Hematopoietic, endothelial and mesenchymal progenitor cells can be isolated simultaneously from human BM by cell sorting based on ALDH activity and may be useful in several cell therapy applications. Expand
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TLDR
These studies suggested that in particular the expression of ALDH delineated distinct CD34+ stem cell and progenitor compartments may provide a means to explore normal and malignant processes associated with myeloid and lymphoid development. Expand
Dual SP/ALDH Functionalities Refine the Human Hematopoietic Lin−CD34+CD38− Stem/Progenitor Cell Compartment
TLDR
This study shows that the coexpression of SP and ALDH markers refines the Lin−CD34+CD38− hematopoietic compartment and identifies an SP/ALDHBr cell subset enriched in quiescent primitive HSCs/HPCs. Expand
The combined use of Hoechst efflux ability and aldehyde dehydrogenase activity to identify murine and human hematopoietic stem cells.
TLDR
It is demonstrated that human Lin(-)/CD34(-)/ALDH(+) cells are capable of long-term repopulation and Hoechst exclusion ability does not seem to be the method of choice for the isolation of human hematopoietic stem cells. Expand
Expansion and preservation of the functional activity of adult hematopoietic stem cells cultured ex vivo with a histone deacetylase inhibitor
TLDR
Grafts harvested from VPA‐treated cultures were able to engraft in immune‐deficient mice and, importantly, to generate cellular progeny belonging to each hematopoietic lineage in similar proportion to that observed with unmanipulated CD34+ cells. Expand
Aldehyde dehydrogenase activity identifies a population of human skeletal muscle cells with high myogenic capacities.
TLDR
The results suggest that ALDH activity is a novel marker for a population of new human skeletal muscle progenitors presenting a potential for cell biology and cell therapy. Expand
Umbilical Cord Blood‐Derived Aldehyde Dehydrogenase‐Expressing Progenitor Cells Promote Recovery from Acute Ischemic Injury
TLDR
This work provides preclinical justification for the development of therapeutic strategies to treat ischemic diseases using UCB‐derived ALDHhi mixed progenitor cells, a readily available population of proangiogenic progenitors that promote vascular regeneration. Expand
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Observations indicate that fractionating human hematopoietic stem cells on the basis of aldehyde dehydrogenase activity using BAAA is an effective method for isolating primitive humanHematopOietic progenitors from other tissues as well. Expand
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It is demonstrated that human CD34(+/CD38(-) cells can generate CD45(+)/CD34(-) progeny in a long-term xenograft model and that those CD45 (+) / CD34 (+) cells can regenerate CD34-+) progeny following secondary transplantation and expression of CD34 can be reversible on reconstituting human hematopoietic stem cells. Expand
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TLDR
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