Functional characterization of genes involved in alkane oxidation by Pseudomonas aeruginosa

@article{Smits2003FunctionalCO,
  title={Functional characterization of genes involved in alkane oxidation by Pseudomonas aeruginosa},
  author={Theo H. M. Smits and Bernard Witholt and Jan B. van Beilen},
  journal={Antonie van Leeuwenhoek},
  year={2003},
  volume={84},
  pages={193-200}
}
Most clinical isolates identified as Pseudomonas aeruginosa grow on long-chain n-alkanes, while environmental P. aeruginosa isolates often grow on medium- as well as long-chain n-alkanes. Heterologous expression showed that the two alkane hydroxylase homologs of P. aeruginosa PAO1 (AlkB1 and AlkB2) oxidize C12-C16 n-alkanes, while two rubredoxin (RubA1 and RubA2) and a rubredoxin reductase (RubB) homologs can replace their P. putida GPo1 counterparts in n-octane oxidation. The two long-chain… CONTINUE READING