Functional characterization of an anaerobic benzene-degrading enrichment culture by DNA stable isotope probing.

@article{Herrmann2010FunctionalCO,
  title={Functional characterization of an anaerobic benzene-degrading enrichment culture by DNA stable isotope probing.},
  author={Steffi Herrmann and Sabine Kleinsteuber and Antonis Chatzinotas and Steffen Kuppardt and Tillmann Lueders and Hans Hermann Richnow and Carsten Vogt},
  journal={Environmental microbiology},
  year={2010},
  volume={12 2},
  pages={
          401-11
        }
}
The flow of carbon under sulfate-reducing conditions within a benzene-mineralizing enrichment culture was analysed using fully labelled [13C6]-benzene. Over 180 days of incubation, 95% of added 13C-benzene was released as 13C-carbon dioxide. DNA extracted from cultures that had degraded different amounts of unlabelled or 13C-labelled benzene was centrifuged in CsCl density gradients to identify 13C-benzene-assimilating organisms by density-resolved terminal restriction fragment length… CONTINUE READING
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