Functional characterization of a salivary apyrase from the sand fly, Phlebotomus duboscqi, a vector of Leishmania major.

@article{Hamasaki2009FunctionalCO,
  title={Functional characterization of a salivary apyrase from the sand fly, Phlebotomus duboscqi, a vector of Leishmania major.},
  author={Ryoichi Hamasaki and Hirotomo Kato and Yoshimi Terayama and Hiroyuki Iwata and Jesus G Valenzuela},
  journal={Journal of insect physiology},
  year={2009},
  volume={55 11},
  pages={1044-9}
}
Two transcripts coding for proteins homologous to apyrases were identified by massive sequencing of a Phlebotomus (P.) duboscqi salivary gland cDNA library. The sequence analysis revealed that the amino acids important for enzymatic activity including nucleotidase activity and the binding of calcium and nucleotides were well conserved in these molecules. A recombinant P. duboscqi salivary apyrase was expressed in Escherichia coli and purified. The resulting protein efficiently hydrolyzed ADP… CONTINUE READING

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