Functional characterization of PmHS1, a Pasteurella multocida heparosan synthase.

@article{Kane2006FunctionalCO,
  title={Functional characterization of PmHS1, a Pasteurella multocida heparosan synthase.},
  author={Tasha A Kane and Carissa L White and Paul L Deangelis},
  journal={The Journal of biological chemistry},
  year={2006},
  volume={281 44},
  pages={33192-7}
}
Heparosan synthase 1 (PmHS1) from Pasteurella multocida Type D is a dual action glycosyltransferase enzyme that transfers monosaccharide units from uridine diphospho (UDP) sugar precursors to form the polysaccharide heparosan (N-acetylheparosan), which is composed of alternating (-alpha4-GlcNAc-beta1,4-GlcUA-1-) repeats. We have used molecular genetic means to remove regions nonessential for catalytic activity from the amino- and the carboxyl-terminal regions as well as characterized the… CONTINUE READING