Functional characterization of NIPA2, a selective Mg2+ transporter.

  title={Functional characterization of NIPA2, a selective Mg2+ transporter.},
  author={Angela Goytain and Rochelle M Hines and Gary A. Quamme},
  journal={American journal of physiology. Cell physiology},
  volume={295 4},
We used microarray analysis to identify renal cell transcripts that were upregulated with low magnesium. One transcript, identified as NIPA2 (nonimprinted in Prader-Willi/Angelman syndrome) subtype 2, was increased over twofold relative to cells cultured in normal magnesium. The deduced sequence comprises 129 amino acids with 8 predicted transmembrane regions. As the secondary structure of NIPA2 conformed to a membrane transport protein, we expressed it in Xenopus oocytes and determined that it… CONTINUE READING