Functional characterization of NADP-dependent isocitrate dehydrogenase isozymes from Trypanosoma cruzi.

@article{Leroux2011FunctionalCO,
  title={Functional characterization of NADP-dependent isocitrate dehydrogenase isozymes from Trypanosoma cruzi.},
  author={Alejandro E. Leroux and Dante Abel Maugeri and Juan Jos{\'e} Cazzulo and Cristina Nowicki},
  journal={Molecular and biochemical parasitology},
  year={2011},
  volume={177 1},
  pages={
          61-4
        }
}
Trypanosoma cruzi exhibits two putative isocitrate dehydrogenases (IDHs). Both idh genes were cloned and the recombinant enzymes expressed in Escherichia coli. Our results showed that T. cruzi IDHs are strictly dependent on NADP(+) and display apparent affinities towards isocitrate and the coenzyme in the low micromolar range. In T. cruzi, IDHs are cytosolic and mitochondrial enzymes, and there is no evidence for the typical Krebs cycle-related NAD-dependent IDH. Hence, like in Trypanosoma… CONTINUE READING

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