Functional characterization of HIV-1 Nef mutants in the context of viral infection.

  title={Functional characterization of HIV-1 Nef mutants in the context of viral infection.},
  author={O. Fackler and A. Moris and Nadine Tibroni and Simone I. Giese and B. Glass and O. Schwartz and H. Kr{\"a}usslich},
  volume={351 2},
  • O. Fackler, A. Moris, +4 authors H. Kräusslich
  • Published 2006
  • Biology, Medicine
  • Virology
  • Nef is an important pathogenesis factor of HIV-1 with a multitude of effector functions. We have designed a broad panel of isogenic viruses encoding defined mutants of HIV-1(SF2) Nef and analyzed their biological activity in the context of productive HIV-1 infection. Analysis of subcellular localization, virion incorporation, downregulation of cell surface CD4 and MHC-I, enhancement of virion infectivity and facilitation of HIV replication in primary human T lymphocytes mostly confirmed the… CONTINUE READING
    92 Citations

    Topics from this paper.

    In vivo analysis of highly conserved Nef activities in HIV-1 replication and pathogenesis
    • 22
    CD4 downregulation by the human immunodeficiency virus type 1 Nef protein is dispensable for optimal output and functionality of viral particles in primary T cells.
    • 13
    • Highly Influenced
    MLV glycosylated-Gag is an infectivity factor that rescues Nef-deficient HIV-1
    • M. Pizzato
    • Biology, Medicine
    • Proceedings of the National Academy of Sciences
    • 2010
    • 52
    • PDF
    Dynamic range of Nef functions in chronic HIV-1 infection.
    • 32
    • Highly Influenced
    Nef enhances HIV-1 infectivity via association with the virus assembly complex.
    • 42
    • Highly Influenced
    Dynamin 2 is required for the enhancement of HIV-1 infectivity by Nef
    • 124
    • PDF
    Clearly different mechanisms of enhancement of short-lived Nef-mediated viral infectivity between SIV and HIV-1
    • 1


    Human Immunodeficiency Virus Type 1 Nef-Mediated Downregulation of CD4 Correlates with Nef Enhancement of Viral Pathogenesis
    • 100
    • PDF
    Nef stimulates human immunodeficiency virus type 1 proviral DNA synthesis.
    • 401
    • PDF
    Nef association with human immunodeficiency virus type 1 virions and cleavage by the viral protease.
    • 103
    • PDF
    Inhibition of HIV-1 progeny virion release by cell-surface CD4 is relieved by expression of the viral Nef protein
    • 270
    Nef Does Not Affect the Efficiency of Human Immunodeficiency Virus Type 1 Fusion with Target Cells
    • 90
    • PDF
    The importance of nef in the induction of human immunodeficiency virus type 1 replication from primary quiescent CD4 lymphocytes
    • 416
    • PDF
    The Proteolytic Cleavage of Human Immunodeficiency Virus Type 1 Nef Does Not Correlate with Its Ability To Stimulate Virion Infectivity
    • 56
    • PDF