Functional characterization of FaCCD1: a carotenoid cleavage dioxygenase from strawberry involved in lutein degradation during fruit ripening.

@article{GarcaLimones2008FunctionalCO,
  title={Functional characterization of FaCCD1: a carotenoid cleavage dioxygenase from strawberry involved in lutein degradation during fruit ripening.},
  author={Carmen Garc{\'i}a-Limones and Kerstin Schn{\"a}bele and Rosario Blanco-Portales and M Luz Bellido and J Caballero and Wilfried Schwab and J. A. Mu{\~n}oz-Blanco},
  journal={Journal of agricultural and food chemistry},
  year={2008},
  volume={56 19},
  pages={
          9277-85
        }
}
A gene encoding a carotenoid cleavage dioxygenase class 1 enzyme (FaCCD1) was identified among a strawberry fruit expressed sequence tag collection. The full-length cDNA was isolated, and the expression profiles along fruit receptacle development and ripening, determined by quantitative real time polymerase chain reaction, showed that FaCCD1 is a ripening-related gene that reaches its maximal level of expression in the red fully ripe stage. FaCCD1 was expressed in Escherichia coli, and the… CONTINUE READING
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