Functional characteristics of leukotriene C4- and D4-metabolizing enzymes (gamma-glutamyl transpeptidase, dipeptidase) within human plasma.

@article{Kller1985FunctionalCO,
  title={Functional characteristics of leukotriene C4- and D4-metabolizing enzymes (gamma-glutamyl transpeptidase, dipeptidase) within human plasma.},
  author={Manfred K{\"o}ller and Wolfgang König and Joachim Brom and Klaus Dieter Dr. Bremm and Wolfgang Schönfeld and J. Kn{\"o}ller},
  journal={Biochimica et biophysica acta},
  year={1985},
  volume={836 1},
  pages={
          56-62
        }
}
Several properties of the leukotriene C4- and leukotriene D4-metabolizing enzymes within human plasma were studied after fractionation of the plasma proteins using ammonium sulfate precipitation. Leukotriene D4-metabolizing enzymes were widely distributed among the fractions obtained. They showed different pH optima (pH 6.5, pH 7.0 and pH greater than or equal to 8.5) and revealed a different degree of thermal stability. The results indicate the presence of more than one enzyme in plasma which… Expand
13 Citations
Release and Functional Characterization of the Leukotriene D4‐Metabolizing Enzyme (Dipeptidase) from Human Polymorphonuclear Leucocytes
TLDR
The LTD4‐dipeptidase activity can also be released from other cell types, but the enzyme activity from human PMN exceeded that of other cells (e.g. human lymphocytes/monocytes and basophils (LMB) and human lung cell suspension). Expand
Oxidative Metabolism of Leukotriene E4 by Rat Hepatocytes a
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It is now widely appreciated that LTC, rapidly undergoes sequential peptide cleavage reactions to LTD, and LTE, which can occur in plasma as well as in tissues due to the ubiquitous nature of gamma-glutamyl transpeptidase, and the sulfidopeptide leukotrienes are known to undergo rapid metabolism. Expand
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The obvious differences in gamma-GT activities demonstrated here may reflect different biochemical capacities of these cell types, related to their distinct functions in the immune system. Expand
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The degradation of imipenem in vitro was analyzed using reversed-phase HPLC and the stability of the antibiotic within serum was improved by 13.9% in the presence of EDTA (10 mM) indicating a possible involvement of serum dipeptidases in the systemic breakdown of the imipanem molecule. Expand
Conversion rate of leukotriene C4 to Leukotriene E4 is decreased in children with asthma
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The result indicates that the conversion rate of LTC4 to LTE4 may contribute to the pathogenesis of childhood asthma. Expand
Heat shock induces alterations of the lipoxygenase pathway in human polymorphonuclear granulocytes.
TLDR
The chemiluminescence response induced by opsonized zymosan and the chemotaxis against C5a and LTB4 was clearly inhibited after heat shock treatment, and enzyme activities of the heat treated PMNs the protein kinase C activities were enhanced whereas the LTD4-dipeptidase and the L TB4-omega-hydroxylase were not affected. Expand
Generation and Metabolism of Leukotrienes and Release of Histamine from Human Dispersed Tonsillar Cells
TLDR
These data clearly demonstrate the generation and metabolism of the 54‐lipoxygenase products LTB4 and LTC4 as well as the release of histamine from human dispersed tonsillar cells, suggesting that they have a modulatory function with respect to the inflammatory potential at local sites. Expand
Studies on the Release of Lipoxygenase Products from Granulocytes of Severely Burned Patients
In recent years major interest has been addressed to the functional behavior of polymorphonuclear granulocytes (PMNs) in patients suffering from severe burns. It was observed that serious PMNExpand
Metabolism of leukotriene B4 by polymorphonuclear granulocytes of severely burned patients.
TLDR
The data suggest a modulation of the enzymatic activities involved in omega-oxidation of LTB4 (isoenzymes of cytochrome P-450) and an unidentified compound which was derived from L TB4 is suggested. Expand
Leukotriene C4 in Children with Atopic Asthma II. Plasma Levels in Bronchial Challenge with Specific Allergen
  • E. Iwasaki
  • Medicine
  • Acta paediatrica Japonica : Overseas edition
  • 1989
TLDR
The in vivo release of LTC4 following exposure to a specific allergen suggests that leukotrienes are important in the pathogenesis of asthma. Expand
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