Functional characterisation and application of an ex vivo perfusion-superfusion system in murine airways.

@article{Cheah2017FunctionalCA,
  title={Functional characterisation and application of an ex vivo perfusion-superfusion system in murine airways.},
  author={Esther Y Cheah and Tracy S Mann and Philip C. Burcham and Peter J. Henry},
  journal={Journal of pharmacological and toxicological methods},
  year={2017},
  volume={84},
  pages={66-77}
}
INTRODUCTION The aim of this study was to develop two dynamic ex vivo airway explant systems, a perfusion-superfusion system and a ventilation-superfusion system, for the study of toxic airborne substances, such as the prevalent smoke constituent acrolein. METHODS Mouse isolated tracheal segments were perfused with physiological media or ventilated with humidified air at 37°C to mimic dynamic flow conditions, and superfused with media over the exterior surface. At selected time points, the… CONTINUE READING

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