Functional cdc25C dual-specificity phosphatase is required for S-phase entry in human cells.

@article{Turowski2003FunctionalCD,
  title={Functional cdc25C dual-specificity phosphatase is required for S-phase entry in human cells.},
  author={Patric Turowski and Celine Franckhauser and May C Morris and Philippe Vaglio and Anne Fernandez and Ned J. C. Lamb},
  journal={Molecular biology of the cell},
  year={2003},
  volume={14 7},
  pages={
          2984-98
        }
}
In view of the common regulatory mechanism that induces transcription of the mitotic phosphatase cdc25C and cyclin A at the beginning of S-phase, we investigated whether cdc25C was required for S-phase transit. Here, we show that in both nontransformed human fibroblasts and HeLa cells, cdc25C protein levels significantly increased concomitant with S-phase onset and cyclin A synthesis. Activity measurements on immunoprecipitates from synchronized HeLa cells revealed a sharp rise in cdc25C… CONTINUE READING