Functional bioengineered corneal epithelial sheet grafts from corneal stem cells expanded ex vivo on a temperature-responsive cell culture surface.

@article{Nishida2004FunctionalBC,
  title={Functional bioengineered corneal epithelial sheet grafts from corneal stem cells expanded ex vivo on a temperature-responsive cell culture surface.},
  author={Kohji Nishida and Masayuki Yamato and Yasutaka Hayashida and Katsuhiko Watanabe and Naoyuki Maeda and Hitoshi Watanabe and Kazuaki Yamamoto and Shigeru Nagai and Akihiko Kikuchi and Yasuo Tano and T. Okano},
  journal={Transplantation},
  year={2004},
  volume={77 3},
  pages={379-85}
}
BACKGROUND Limbal stem-cell deficiency by ocular trauma or diseases causes corneal opacification and visual loss. Recent attempts have been made to fabricate corneal epithelial graft constructs, but the technology is still evolving. We have developed a novel cell-sheet manipulation technology using temperature-responsive culture surfaces to generate functional, cultivated corneal epithelial cell sheet grafts. METHODS Human or rabbit limbal stem cells were cocultured with mitomycin C-treated… CONTINUE READING
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