Functional association of U2 snRNP with the ATP-independent spliceosomal complex E.

@article{Das2000FunctionalAO,
  title={Functional association of U2 snRNP with the ATP-independent spliceosomal complex E.},
  author={Rita Das and Zhaolan Zhou and Robin Reed},
  journal={Molecular cell},
  year={2000},
  volume={5 5},
  pages={779-87}
}
In the current model for spliceosome assembly, U1 snRNP binds to the 5' splice site in the E complex followed by ATP-dependent binding of U2 snRNP to the branchpoint sequence (BPS) in the A complex. Here we report the characterization of highly purified, functional E complex. We provide evidence that this complex contains functional U2 snRNP and that this snRNP is required for E complex assembly. The BPS is not required for U2 snRNP binding in the E complex. These data suggest a model for… CONTINUE READING

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