Functional assessment of centrosomes of spermatozoa and spermatids microinjected into rabbit oocytes.

@article{Tachibana2009FunctionalAO,
  title={Functional assessment of centrosomes of spermatozoa and spermatids microinjected into rabbit oocytes.},
  author={Masahito Tachibana and Yukihiro Terada and Narumi Ogonuki and Tomohisa Ugajin and Atsuo Ogura and Takashi Murakami and Nobuo Yaegashi and Kunihiro Okamura},
  journal={Molecular reproduction and development},
  year={2009},
  volume={76 3},
  pages={270-7}
}
Although intracytoplasmic sperm injection (ICSI) is a widely used assisted reproductive technique, the fertilization rates and pregnancy rates of immature spermatids especially in round spermatid injection (ROSI) remain very low. During mammalian fertilization, the sperm typically introduces its own centrosome which then acts as a microtubule organizing center (MTOC) and is essential for the male and female genome union. In order to evaluate the function of immature germ cell centrosomes, we… CONTINUE READING
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