Functional assembly of minicellulosomes on the Saccharomyces cerevisiae cell surface for cellulose hydrolysis and ethanol production.

@article{Tsai2009FunctionalAO,
  title={Functional assembly of minicellulosomes on the Saccharomyces cerevisiae cell surface for cellulose hydrolysis and ethanol production.},
  author={Shen-Long Tsai and Jeongseok Oh and Shailendra Singh and Ruizhen Chen and Wilfred Chen},
  journal={Applied and environmental microbiology},
  year={2009},
  volume={75 19},
  pages={
          6087-93
        }
}
We demonstrated the functional display of a miniscaffoldin on the Saccharomyces cerevisiae cell surface consisting of three divergent cohesin domains from Clostridium thermocellum (t), Clostridium cellulolyticum (c), and Ruminococcus flavefaciens (f). Incubation with Escherichia coli lysates containing an endoglucanase (CelA) fused with a dockerin domain from C. thermocellum (At), an exoglucanase (CelE) from C. cellulolyticum fused with a dockerin domain from the same species (Ec), and an… CONTINUE READING

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