Functional assay for BRCA1: mutagenesis of the COOH-terminal region reveals critical residues for transcription activation.

@article{Hayes2000FunctionalAF,
  title={Functional assay for BRCA1: mutagenesis of the COOH-terminal region reveals critical residues for transcription activation.},
  author={Finbarr Hayes and Charmagne S Cayanan and Daniela Barill{\`a} and Alvaro N. A. Monteiro},
  journal={Cancer research},
  year={2000},
  volume={60 9},
  pages={2411-8}
}
The breast and ovarian cancer susceptibility gene product BRCA1 is a tumor suppressor, but its precise biochemical function remains unknown. The BRCA1 COOH terminus acts as a transcription activation domain, and germ-line cancer- predisposing mutations in this region abolish transcription activation, whereas benign polymorphisms do not. These results raise the possibility that loss of transcription activation by BRCA1 is crucial for oncogenesis. Therefore, identification of residues involved in… CONTINUE READING