Functional and shunt states of bacteriorhodopsin resolved by 250 GHz dynamic nuclear polarization-enhanced solid-state NMR.

@article{Bajaj2009FunctionalAS,
  title={Functional and shunt states of bacteriorhodopsin resolved by 250 GHz dynamic nuclear polarization-enhanced solid-state NMR.},
  author={Vikram S. Bajaj and Melody L. Mak-Jurkauskas and Marina L Belenky and Judith Herzfeld and Robert G Griffin},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={2009},
  volume={106 23},
  pages={
          9244-9
        }
}
Observation and structural studies of reaction intermediates of proteins are challenging because of the mixtures of states usually present at low concentrations. Here, we use a 250 GHz gyrotron (cyclotron resonance maser) and cryogenic temperatures to perform high-frequency dynamic nuclear polarization (DNP) NMR experiments that enhance sensitivity in magic-angle spinning NMR spectra of cryo-trapped photocycle intermediates of bacteriorhodopsin (bR) by a factor of approximately 90… CONTINUE READING
Highly Cited
This paper has 93 citations. REVIEW CITATIONS

From This Paper

Figures, tables, and topics from this paper.

Citations

Publications citing this paper.
Showing 1-10 of 72 citations

93 Citations

051015'10'12'14'16'18
Citations per Year
Semantic Scholar estimates that this publication has 93 citations based on the available data.

See our FAQ for additional information.

References

Publications referenced by this paper.
Showing 1-10 of 40 references

Early and late M intermediates in the bacteriorhodopsin photocycle: A solid-state NMR study

  • JG Hu
  • Biochemistry
  • 1998
Highly Influential
4 Excerpts