Functional and physical characteristics of rat Leydig cell populations isolated by metrizamide and Percoll gradient centrifugation.

@article{Georgiou1987FunctionalAP,
  title={Functional and physical characteristics of rat Leydig cell populations isolated by metrizamide and Percoll gradient centrifugation.},
  author={Markos Georgiou and A. Howard Payne},
  journal={Biology of reproduction},
  year={1987},
  volume={37 2},
  pages={
          335-41
        }
}
The physical and functional properties of Leydig cell populations obtained by centrifugation of testicular cells in two different density gradient media, Percoll and Metrizamide, were compared. Percoll-gradient centrifugation yielded two Leydig cell bands (Peak I and Peak II) that were comparable, as to their density and testosterone-producing capacity, to the respective Leydig cell bands, Population I and Population II, isolated in a Metrizamide gradient. The denser Leydig cell band (II) had a… CONTINUE READING
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