Functional and molecular characterization of a glycosomal PPi-dependent enzyme in trypanosomatids: pyruvate, phosphate dikinase.

@article{Bringaud1998FunctionalAM,
  title={Functional and molecular characterization of a glycosomal PPi-dependent enzyme in trypanosomatids: pyruvate, phosphate dikinase.},
  author={Fr{\'e}d{\'e}ric Bringaud and Dominique Baltz and Th{\'e}o Baltz},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1998},
  volume={95 14},
  pages={7963-8}
}
Trypanosomatids are parasitic protists that have an ATP-dependent glycolysis with no indication of PPi-dependent metabolism. Most of the glycolysis takes place in peroxisome-like organelles, the glycosomes. We characterized in Trypanosoma brucei a single-copy gene encoding a PPi-dependent enzyme, pyruvate, phosphate dikinase (PPDK), which was expressed functionally in Escherichia coli. Specific antibodies detected a 100-kDa protein in procyclic forms but not in mammalian forms of T. brucei… CONTINUE READING

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