Functional and Molecular Characterization of a Volume-activated Chloride Channel in Rabbit Corneal Epithelial Cells

@article{AlNakkash2004FunctionalAM,
  title={Functional and Molecular Characterization of a Volume-activated Chloride Channel in Rabbit Corneal Epithelial Cells},
  author={L. Al-Nakkash and Pavel Iserovich and Miguel Coca-Prados and H. Yang and Peter Sol Reinach},
  journal={The Journal of Membrane Biology},
  year={2004},
  volume={201},
  pages={41-49}
}
We characterized the functional and molecular properties of a volume-regulated anion channel (VRAC) in SV40-immortalized rabbit corneal epithelial cells (tRCE), since they mediate a robust regulatory volume decrease (RVD) response during exposure to a hypotonic challenge. Whole-cell patch clamp-monitored chloride currents and light- scattering measurements evaluated temporal cell- volume responsiveness to hypoosmotic challenges. Exposure to 200 mOsm medium elicited an outwardly-rectifying… CONTINUE READING

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