Functional analysis of very long-chain fatty acid elongase gene, HpELO2, in the methylotrophic yeast Hansenula polymorpha

@article{Prasitchoke2007FunctionalAO,
  title={Functional analysis of very long-chain fatty acid elongase gene, HpELO2, in the methylotrophic yeast Hansenula polymorpha},
  author={Phatthanon Prasitchoke and Yoshinobu Kaneko and Minetaka Sugiyama and Takeshi Bamba and Eiichiro Fukusaki and Akio Kobayashi and S. Harashima},
  journal={Applied Microbiology and Biotechnology},
  year={2007},
  volume={76},
  pages={417-427}
}
We describe the cloning and functional characterization of the fatty acid elongase gene HpELO2, a homologue of the HpELO1 gene required for the production of C24:0 in the yeast Hansenula polymorpha. The open reading frame (ORF) of HpELO2 consists of 1,035 bp, encoding 344 amino acids, sharing about 65% identity with that of Saccharomyces cerevisiae Elo2. Expression of HpELO2 rescued the lethality of the S. cerevisiae elo2Δ elo3Δ double disruptant. An accumulation of C18:0 and a significant… CONTINUE READING