Functional analysis of the sialyltransferase complexes in Escherichia coli K1 and K92.

@article{Steenbergen1992FunctionalAO,
  title={Functional analysis of the sialyltransferase complexes in Escherichia coli K1 and K92.},
  author={Susan M. Steenbergen and T J Wrona and Eric R. Vimr},
  journal={Journal of bacteriology},
  year={1992},
  volume={174 4},
  pages={1099-108}
}
The polysialyltransferase (polyST) structural gene, neuS, for poly alpha 2,8sialic acid (PSA) capsule synthesis in Escherichia coli K1 was previously mapped near the kps region 1 and 2 junction (S. M. Steenbergen and E. R. Vimr, Mol. Microbiol. 4:603-611, 1990). Present Southern and colony blot hybridization results confirmed that neuS was a region 2 locus and indicated apparent homology with neuS from E. coli K92, bacteria that synthesize a sialyl alpha 2,8-2,9-linked polymer. A K1- mutant… CONTINUE READING

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