Functional analysis of the human N-acetyltransferase 1 major promoter: quantitation of tissue expression and identification of critical sequence elements.

@article{Husain2007FunctionalAO,
  title={Functional analysis of the human N-acetyltransferase 1 major promoter: quantitation of tissue expression and identification of critical sequence elements.},
  author={Anwar Husain and Xiaoyan Zhang and Mark A. Doll and J Christopher States and David F. Barker and David W Hein},
  journal={Drug metabolism and disposition: the biological fate of chemicals},
  year={2007},
  volume={35 9},
  pages={1649-56}
}
Arylamine N-acetyltransferase 1 (NAT1) plays an important role in the biotransformation of xenobiotics, and genetic variants have been implicated in susceptibility to cancer and birth defects. A specific and quantitative reverse transcription-polymerase chain reaction assay for transcription from the major NAT1 promoter detected high expression with limited variability in human tissues. A 213-base pair (bp) minimal promoter was identified by transfection of luciferase reporter constructs into… CONTINUE READING