Functional analysis of nucleosome assembly protein, NAP-1. The negatively charged COOH-terminal region is not necessary for the intrinsic assembly activity.

@article{FujiiNakata1992FunctionalAO,
  title={Functional analysis of nucleosome assembly protein, NAP-1. The negatively charged COOH-terminal region is not necessary for the intrinsic assembly activity.},
  author={T Fujii-Nakata and Yukio Ishimi and A. Okuda and Akira Kikuchi},
  journal={The Journal of biological chemistry},
  year={1992},
  volume={267 29},
  pages={20980-6}
}
A nucleosome assembly protein (NAP-1) of Saccharomyces cerevisiae facilitates the association of histones with DNA to form nucleosomes in vitro at physiological ionic conditions. The cloned gene was expressed in Escherichia coli using a T7 expression system, and the protein (417 amino acid residues) was purified by Mono Q column chromatography. Various deletion fragments of NAP-1 protein were also produced, and their nucleosome assembly activity was examined by supercoiling assay. The internal… CONTINUE READING

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