Functional analysis by site-directed mutagenesis of individual amino acid residues in the flavin domain of Neurospora crassa nitrate reductase

@article{Gonzlez1995FunctionalAB,
  title={Functional analysis by site-directed mutagenesis of individual amino acid residues in the flavin domain of Neurospora crassa nitrate reductase},
  author={Celedonio Gonz{\'a}lez and N{\'e}lida Brito and George A. Marzluf},
  journal={Molecular and General Genetics MGG},
  year={1995},
  volume={249},
  pages={456-464}
}
Nitrate reductase of Neurospora crassa is a complex multi-redox protein composed of two identical subunits, each of which contains three distinct domains, an amino-terminal domain that contains a molybdopterin cofactor, a central heme-containing domain, and a carboxy-terminal domain which binds a flavin and a pyridine nucleotide cofactor. The flavin domain of nitrate reductase appears to have structural and functional similarity to ferredoxin NADPH reductase (FNR). Using the crystal structure… CONTINUE READING