Acute alveolar injury (AAI) was induced in dogs by injection of N-nitroso-N-methylurethane. Two to 20 days after injection, alveolar lavage phospholipids were quantified. Lavage surfactant was partially purified by centrifugation (27,000 g for 2 h), and further purified by centrifugation in NaBr density gradient (100,000 g for 4 h). Phospholipids, neutral… (More)
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