Zika Virus Subgenomic Flavivirus RNA Generation Requires Cooperativity between Duplicated RNA Structures That Are Essential for Productive Infection in Human Cells
- BiologyJournal of Virology
It is found that two RNA structures of the viral 3′ UTR operate in a cooperative manner to produce two species of sfRNAs and that the deletion of these elements has a profoundly different impact on viral replication in the two hosts.
Zika virus noncoding RNA suppresses apoptosis and is required for virus transmission by mosquitoes
- Biology, MedicineNature Communications
It is shown that sfRNA affects virus-induced apoptosis and dissemination of ZIKV in Aedes aegypti mosquitoes, suggesting a role of sf RNA in Zika virus replication and transmission.
Inhibition of Zika Virus Replication by Silvestrol
In both infected A549 cells and primary human hepatocytes, silvestrol has the potential to exert a significant inhibition of ZIKV replication for both analyzed strains, even though the ancestor strain from Uganda is less sensitive tosilvestrol.
Biochemistry and Molecular Biology of Flaviviruses.
- BiologyChemical reviews
This review examines the molecular biology of flaviviruses touching on the structure and function of viral components and how these interact with host factors, and highlights the role of a noncoding RNA produced by flavIViruses to impair antiviral host immune responses.
Circularization of flavivirus genomic RNA inhibits de novo translation initiation
- BiologyNucleic acids research
This work demonstrates that circularization inhibits de novo translation initiation on ZIKV and DENV RNA, whilst the linear conformation is translation-competent and defines opposing roles for linear and circular conformations of the flavivirus genome.
The dinucleotide composition of the Zika virus genome is shaped by conflicting evolutionary pressures in mammalian hosts and mosquito vectors
- BiologyPLoS biology
The evolutionary pressure on the CpG dinucleotides of viral genomes in arthropod vectors directly opposes the pressure present in vertebrate host cells, which provides evidence that an adaptive compromise is required for arbovirus transmission.
Zika virus: Molecular responses and tissue tropism in the mammalian host
- BiologyReviews in medical virology
An update on ZikV research models and mammalian host responses with respect to ZIKV tissue infection is provided.
Zika virus: A review article
Zika virus, a flavivirus which is spread by Aedes mosquitoes, was considered as innocent pathogen while restricted to the African and Asian population. However, after reaching the Americas in March…
Evasion of Innate and Intrinsic Antiviral Pathways by the Zika Virus
- Biology, MedicineViruses
This work describes emerging evidence that indicated the contribution of several cell-intrinsic mechanisms to an effective restriction of ZIKV infection, such as nonsense-mediated mRNA decay, stress granule formation, and “reticulophagy”, a type of selective autophagy.
Evaluation of Conserved RNA Secondary Structures within and between Geographic Lineages of Zika Virus
This work proposes these structures as candidates for further experimental validation to establish their potential role in vital functions of the viral cycle of ZIKV and their possible associations with the singularities of different outbreaks that lead to ZikV populations to acquire nucleotide substitutions, which is evidence of the local structure genome differentiation.
SHOWING 1-10 OF 186 REFERENCES
Full Genome Sequence and sfRNA Interferon Antagonist Activity of Zika Virus from Recife, Brazil
- Biology, MedicinePLoS neglected tropical diseases
The full-length genome sequence including non-coding regions of a South American ZIKV isolate from a patient with classical symptoms will support efforts to develop genetic tools for this virus.
Noncoding Subgenomic Flavivirus RNA: Multiple Functions in West Nile Virus Pathogenesis and Modulation of Host Responses
Flaviviruses are a large group of positive strand RNA viruses transmitted by arthropods that include many human pathogens such as West Nile virus (WNV), Japanese encephalitis virus (JEV), yellow…
Nucleotide composition of the Zika virus RNA genome and its codon usage
- BiologyVirology Journal
The ZIKV RNA genome has a biased nucleotide composition that dictates the codon usage of this flavivirus, which is a general characteristic of the mosquito-borne and tick-borne flaviviruses.
Noncoding Flavivirus RNA Displays RNA Interference Suppressor Activity in Insect and Mammalian Cells
- BiologyJournal of Virology
The results of the present study suggest a novel role for sfRNA, i.e., as a nucleic acid-based regulator of RNAi pathways, a strategy that may be conserved among flaviviruses.
The Structural Basis of Pathogenic Subgenomic Flavivirus RNA (sfRNA) Production
The crystal structure of a complete Xrn1-resistant flaviviral RNA is presented, which contains interwoven pseudoknots within a compact structure that depends on highly conserved nucleotides.
Structure and function of the Zika virus full-length NS5 protein
- Biology, ChemistryNature communications
The crystal structures of full-length NS5 and its polymerase domain at 3.0 Å resolution are reported, which will contribute to future studies on ZikV infection and the development of inhibitors of ZIKV replication.
Noncoding Subgenomic Flavivirus RNA Is Processed by the Mosquito RNA Interference Machinery and Determines West Nile Virus Transmission by Culex pipiens Mosquitoes
- BiologyJournal of Virology
It is demonstrated that sfRNA determines the infection and transmission rates of West Nile virus in Culex pipiens mosquitoes and is the first report to describe a key biological function of s fRNA for flavivirus infection of the arthropod vector, providing an explanation for the strict conservation of sf RNA production.
Functional non-coding RNAs derived from the flavivirus 3' untranslated region.
- BiologyVirus research
Functional Information Stored in the Conserved Structural RNA Domains of Flavivirus Genomes
- BiologyFront. Microbiol.
This review summarizes the knowledge of the features of flavivirus genome functional RNA domains and provides a brief overview of the main achievements reported in the design of antiviral nucleic acid-based drugs targeting functional genomic RNA elements.
Structure and function of the Zika virus full-length NS 5 protein
- Biology, Chemistry
The crystal structures of full-length NS5 and its polymerase domain at 3.0 Å resolution show striking similarities to the NS5 protein of the related Japanese encephalitis virus, which will contribute to future studies on ZikV infection and the development of inhibitors of ZIKV replication.