Functional Modulation of ES-Derived Hepatocyte Lineage Cells via Substrate Compliance Alteration
@article{Li2008FunctionalMO, title={Functional Modulation of ES-Derived Hepatocyte Lineage Cells via Substrate Compliance Alteration}, author={Lulu Li and Nripen S. Sharma and Uday Chippada and Xue Jiang and Rene R Schloss and Martin L. Yarmush and Noshir A. Langrana}, journal={Annals of Biomedical Engineering}, year={2008}, volume={36}, pages={865-876} }
Pluripotent embryonic stem cells represent a promising renewable cell source to generate a variety of differentiated cell types including hepatocyte lineage cells, and may ultimately be incorporated into extracorporeal bioartificial liver devices and cell replacement therapies. Recently, we and others have utilized sodium butyrate to directly differentiate hepatocyte-like cells from murine embryonic stem cells cultured in a monolayer configuration. However, to incorporate stem cell technology…
35 Citations
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References
SHOWING 1-10 OF 34 REFERENCES
Sodium butyrate‐treated embryonic stem cells yield hepatocyte‐like cells expressing a glycolytic phenotype
- BiologyBiotechnology and bioengineering
- 2006
It is shown that Na‐butyrate‐mediated differentiated cells mediate energy metabolism predominantly through glycolysis and this metabolic and mitochondrial characterization can assist in evaluating stem cell differentiation and may prove useful in identifying key regulatory molecules in mediating further differentiation.
Generation of Hepatocyte-Like Cells from Human Embryonic Stem Cells
- Biology, MedicineCell transplantation
- 2003
It is reported here that hES cells can be induced to differentiate into hepatocyte-like cells, and these cells may provide a reliable source of normal human hepatocytes for research and transplantation.
Engineering hepatocyte functional fate through growth factor dynamics: the role of cell morphologic priming.
- Biology, MedicineBiotechnology and bioengineering
- 2001
Insight into the ability of cell shape to "prime" hepatocyte behavior in dynamic microenvironments were elucidated and the possibility of uncoupling and, thus, selectively manipulating, the concerted contributions of GF-induced cellular activation and substrate- and GF- induced cell morphogenesis toward induction of cell function was reported.
Embryoid body-mediated differentiation of mouse embryonic stem cells along a hepatocyte lineage: insights from gene expression profiles.
- BiologyTissue engineering
- 2006
The results of these studies suggest that spontaneous and collagen-mediated differentiation induce cells with the highest levels of ALB expression but mature liver specific genes were only expressed in the spontaneous condition.
Liver stem cells: prospects for treatment of inherited and acquired liver diseases
- Medicine, BiologyExpert opinion on biological therapy
- 2003
The current understanding of these various cell populations is summarized, and possible approaches to their therapeutic use are reviewed, including cell transplantation, bioartificial liver devices (BLDs), gene therapy and administration of exogenous factors to stimulate normal physiological responses to repair.
Mechanochemical manipulation of hepatocyte aggregation can selectively induce or repress liver-specific function.
- BiologyBiotechnology and bioengineering
- 2000
These studies highlight a novel interplay of physicochemical parameters of the culture microenvironment, leading to selective enhancement or repression of differentiated functions of hepatocytes, in concert with the activation of cellular morphogenesis.
Stem cell therapy of the liver— Fusion or fiction?
- Biology, MedicineLiver transplantation : official publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society
- 2004
This review provides an overview of different stem cell populations in distinct models of liver regeneration aimed at unifying diverse views of liver stem cell biology and seeks to clarify the nomenclature of putative liver stem Cell types.
The extracellular matrix coordinately modulates liver transcription factors and hepatocyte morphology
- Biology, EngineeringMolecular and cellular biology
- 1991
It is concluded that the ECM can stimulate cell differentiation by selectively activating transcriptional regulatory factors and that such regulation occurs coordinately with ECM-promoted changes in cell shape.