Functional Modulation of ES-Derived Hepatocyte Lineage Cells via Substrate Compliance Alteration

@article{Li2008FunctionalMO,
  title={Functional Modulation of ES-Derived Hepatocyte Lineage Cells via Substrate Compliance Alteration},
  author={Lulu Li and N. Sharma and Uday Chippada and Xue Jiang and R. Schloss and M. Yarmush and N. Langrana},
  journal={Annals of Biomedical Engineering},
  year={2008},
  volume={36},
  pages={865-876}
}
Pluripotent embryonic stem cells represent a promising renewable cell source to generate a variety of differentiated cell types including hepatocyte lineage cells, and may ultimately be incorporated into extracorporeal bioartificial liver devices and cell replacement therapies. Recently, we and others have utilized sodium butyrate to directly differentiate hepatocyte-like cells from murine embryonic stem cells cultured in a monolayer configuration. However, to incorporate stem cell technology… Expand
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34 Citations
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Methods Citations
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34 Citations

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Citation Type

Neural lineage differentiation of embryonic stem cells within alginate microbeads.
TLDR
In conclusion, it is determined that 2.2% alginate microencapsulation can be optimally adapted to ES neural differentiation and could potentially be adaptable to differentiation of other stem cell types given the correct inducible factors and material properties. Expand
Differentiation of embryonic stem cells into neural lineages in an alginate encapsulation microenvironment
TLDR
A murine embryonic stem cell alginate-poly-l-lysine microencapsulation differentiation system is established that maintains cell viability, is conducive to ES cell differentiation to hepatocyte lineage cells, and sustains differentiated cellular function. Expand
Generation of functional hepatocytes from mouse induced pluripotent stem cells
TLDR
The results indicate that mouse induced pluripotent stem cells can efficiently differentiate into functional hepatocytes in vitro, which may be helpful for the study of liver development and regenerative medicine. Expand
In Vitro Differentiation of Embryonic and Adult Stem Cells into Hepatocytes: State of the Art
TLDR
This review focuses on current strategies to differentiate embryonic, mesenchymal(‐like), and liver stem/progenitor cells into hepatocytes in vitro, and some recommendations are proposed to standardize, optimize, and enrich the in vitro production of hepatocyte‐like cells out of stem/ ProGenitor cells. Expand
Effect of substrate stiffness on early human embryonic stem cell differentiation
TLDR
The utility of PDMS substrates for stem cell propagation and substrate mediated differentiation is evaluated with results indicating that these substrate systems could potentially be used to direct hESC fate towards early mesodermal lineages. Expand
Effects of reconstituted collagen matrix on fates of mouse embryonic stem cells before and after induction for chondrogenic differentiation.
TLDR
Investigation of the effects of reconstituted 3D collagen matrix on the fates of mouse ES cells before and after induction for chondrogenic differentiation suggests the inductive role of collagen matrix in differentiating mES cells toward mesenchymal lineages. Expand
Substrate stiffness affects early differentiation events in embryonic stem cells.
TLDR
A fundamental role for mechanosensing in mammalian development is suggested and the mechanical environment should be taken into consideration when engineering implantable scaffolds or when producing therapeutically relevant cell populations in vitro is illustrated. Expand
Generation of functional hepatic cells from pluripotent stem cells.
TLDR
An updated summary of hepatic differentiation protocols published so far is provided, with a characterization of the hepatic cells generated in vitro and their ability to regenerate damaged livers in vivo following transplantation in pre-clinical liver deficient mouse models. Expand
Encapsulated mesenchymal stromal cells for in vivo transplantation
TLDR
The combined findings support the inclusion of immobilized hMSC in post‐CNS trauma tissue protective therapy, and suggest that conversion of macrophages to the M2 subset is responsible, at least in part, for tissue protection. Expand
Role of epigenetics in liver-specific gene transcription, hepatocyte differentiation and stem cell reprogrammation.
TLDR
The epigenetic control of (liver-specific) gene-transcription and the intricate interplay between chromatin modulation, including histone (de)acetylation and DNA ( de)methylation, and liver-enriched transcription factors are discussed. Expand
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