Functional Metagenomics of a Biostimulated Petroleum-Contaminated Soil Reveals an Extraordinary Diversity of Extradiol Dioxygenases.

@article{TerrnGonzlez2016FunctionalMO,
  title={Functional Metagenomics of a Biostimulated Petroleum-Contaminated Soil Reveals an Extraordinary Diversity of Extradiol Dioxygenases.},
  author={Laura Terr{\'o}n-Gonz{\'a}lez and Guadalupe Mart{\'i}n-Cabello and Manuel Ferrer and Eduardo Santero},
  journal={Applied and environmental microbiology},
  year={2016},
  volume={82 8},
  pages={
          2467-2478
        }
}
  • Laura Terrón-González, Guadalupe Martín-Cabello, +1 author Eduardo Santero
  • Published in
    Applied and environmental…
    2016
  • Biology, Medicine
  • A metagenomic library of a petroleum-contaminated soil was constructed in a fosmid vector that allowed heterologous expression of metagenomic DNA. The library, consisting of 6.5 Gb of metagenomic DNA, was screened for extradiol dioxygenase (Edo) activity using catechol and 2,3-dihydroxybiphenyl as the substrates. Fifty-eight independent clones encoding extradiol dioxygenase activity were identified. Forty-one different Edo-encoding genes were identified. The population of Edo genes was not… CONTINUE READING

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