Functional Interactions between Polydnavirus and Host Cellular Innexins

  title={Functional Interactions between Polydnavirus and Host Cellular Innexins},
  author={Nerissa K. Marziano and Daniel K. Hasegawa and Pauline Phelan and Matthew W Turnbull},
  journal={Journal of Virology},
  pages={10222 - 10229}
ABSTRACT Polydnaviruses are double-stranded DNA viruses associated with some subfamilies of ichneumonoid parasitoid wasps. Polydnavirus virions are delivered during wasp parasitization of a host, and virus gene expression in the host induces alterations of host physiology. Infection of susceptible host caterpillars by the polydnavirus Campoletis sonorensis ichnovirus (CsIV) leads to expression of virus genes, resulting in immune and developmental disruptions. CsIV carries four homologues of… 
Gap junctions: Regulators of cellular immunity and parasite manipulation in the remarkable tripartite relationship involving insects and genome-integrated polydnaviruses
It is shown that VinnexinQ1 and Vinnex inQ2, likewise, form junctions in this heterologous system, and it is demonstrated that the vinnexins interact differentially with the Innexin2 orthologue of an Ichnovirus host, Spodoptera frugiperda.
Polydnavirus Innexins Disrupt Host Cellular Encapsulation and Larval Maturation
A role for Vinnexins in CsIV and more broadly Ichnovirus pathology in infected lepidopteran hosts, particularly in disrupting multicellular developmental and immune physiology is supported.
Ectopic Expression of Virus Innexins in Heliothis virescens Disrupts Hemocyte-Mediated Encapsulation and Host Viability
In vivo expression of two members of a family of Polydnavirus Vinnexin gene family members support that gene family expansion has functional consequences to both PolydNavirus and host wasp success and indicate a need to test gene family member functionality across infected hosts to determine member contribution to host range.
Virus Innexins induce alterations in insect cell and tissue function.
Virus innexin expression in insect cells disrupts cell membrane potential and pH.
Results hint that members of the Vinnexin protein family may affect host bio-electrical phenomena to disrupt host cell physiology, and that the individual proteins of the family may differentially affect host physiology.
Intracellular dynamics of polydnavirus innexin homologues
The findings support that the Vinnexins may disrupt host cell physiology in a protein‐specific manner through altering gap junctional intercellular channel communication, as well as indirectly by affecting multicellular junction characteristics.
The dual life of ichnoviruses.
A Role for Innexin2 and Innexin3 Proteins from Spodoptera litura in Apoptosis
This study showed that Sf9 and Spli221 cells undergo low level apoptosis under normal culture conditions, but not Hi5 cells, which suggests that innexon-promoted apoptosis may be involving the PI3K/Akt signaling pathway.
Extensive Transcription Analysis of the Hyposoter didymator Ichnovirus Genome in Permissive and Non-Permissive Lepidopteran Host Species
Ichnoviruses are large dsDNA viruses that belong to the Polydnaviridae family. They are specifically associated with endoparasitic wasps of the family Ichneumonidae and essential for host


Functional gap junction genes are encoded by insect viruses
Unfolding the evolutionary story of polydnaviruses.
Comparative genomics of mutualistic viruses of Glyptapanteles parasitic wasps
Phylogenetic analyses of the bracoviral genes encoding sugar transporters provides the first robust evidence of a wasp origin for some polydnavirus genes, and it is hypothesized transposable elements, such as those described here, could facilitate transfer of genes between proviral segments and host DNA.
Perspectives on polydnavirus origins and evolution.
Analysis of Virion Structural Components Reveals Vestiges of the Ancestral Ichnovirus Genome
Many thousands of endoparasitic wasp species are known to inject polydnavirus (PDV) particles into their caterpillar host during oviposition, causing immune and developmental dysfunctions that
Iκβ-Related vankyrin Genes in the Campoletis sonorensis Ichnovirus: Temporal and Tissue-Specific Patterns of Expression in Parasitized Heliothis virescens Lepidopteran Hosts
It is hypothesized that PDV vankyrin genes may suppress NF-κβ activity during immune responses and developmental cascades in parasitized lepidopteran hosts of C. sonorensis.
Divergences in Protein Activity and Cellular Localization within the Campoletis sonorensis Ichnovirus Vankyrin Family
ABSTRACT Ichnoviruses (IVs) occur in obligate symbiotic associations with endoparasitic ichneumonid wasps. IVs are injected with eggs during parasitization, where viral infection and gene expression
Polydnavirus infection inhibits translation of specific growth-associated host proteins.
Polydnavirus infection inhibits synthesis of an insect plasma protein, arylphorin.
Injection of purified virus elicited the response observed in naturally parasitized larvae, demonstrating that the effect on arylphorin synthesis is mediated, either directly or indirectly, by polydnavirus gene product(s).
Polydnavirus genomes reflect their dual roles as mutualists and pathogens.