Functional G protein-coupled receptor assays for primary and secondary screening.

@article{Eglen2005FunctionalGP,
  title={Functional G protein-coupled receptor assays for primary and secondary screening.},
  author={R. M. Eglen},
  journal={Combinatorial chemistry & high throughput screening},
  year={2005},
  volume={8 4},
  pages={
          311-8
        }
}
  • R. M. Eglen
  • Published 2005 in
    Combinatorial chemistry & high throughput…
Screening technologies for G protein-coupled receptors comprise two major approaches; homogeneous assays, conducted in microtiter plate formats, and protein redistribution assays that require imaged-based analysis using automated confocal systems. Generally, the former are used in primary screening campaigns for lead identification, while the latter are used in secondary screens for lead optimization. Homogeneous assays measure changes in G protein-coupled receptor second messengers such as… CONTINUE READING