Functional Expression Cloning of Nanog, a Pluripotency Sustaining Factor in Embryonic Stem Cells

  title={Functional Expression Cloning of Nanog, a Pluripotency Sustaining Factor in Embryonic Stem Cells},
  author={Ian Chambers and Douglas Colby and Morag Robertson and Jennifer Nichols and Sonia A Lee and Susan Tweedie and Andrew J.H. Smith},

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Characterisation of the pluripotency determinant Nanog

In this thesis experiments are presented that further the functional characterisation of Nanog, a divergent homeodomain protein with the capacity to direct constitutive self-renewal in absence of otherwise obligatory cytokine stimulation.

A Heterogeneous Expression Pattern for Nanog in Embryonic Stem Cells

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Overexpression of NANOG in human ES cells enables feeder-free growth while inducing primitive ectoderm features

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Nanog safeguards pluripotency and mediates germline development

By genetic deletion, it is shown that, although they are prone to differentiate, embryonic stem cells can self-renew indefinitely in the permanent absence of Nanog, and it is surmised that Nanog stabilizes embryonicstem cells in culture by resisting or reversing alternative gene expression states.



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Self-renewal of pluripotent embryonic stem cells is mediated via activation of STAT3.

Investigation of the role of STAT3 and inducible STAT3F in ES cells growing in the presence of LIF specifically abrogated self-renewal and promoted differentiation establish that STAT3 plays a central role in the maintenance of the pluripotential stem cell phenotype.

Functional gene screening in embryonic stem cells implicates Wnt antagonism in neural differentiation

The use of episomal expression technology for functional evaluation of cDNA clones during ES-cell differentiation in vitro revealed that the Wnt antagonist Sfrp2 stimulates production of neural progenitors and reveals the importance of Wnt signaling in regulating ES- cell lineage diversification.

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The derivation of pluripotent embryonic stem (ES) cells from human blastocysts is described, providing a model to study early human embryology, an investigational tool for discovery of novel growth factors and medicines, and a potential source of cells for use in transplantation therapy.

Molecular mechanism to maintain stem cell renewal of ES cells.

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  • Biology
    Cell structure and function
  • 2001
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