Functional Expression Cloning of Nanog, a Pluripotency Sustaining Factor in Embryonic Stem Cells

  title={Functional Expression Cloning of Nanog, a Pluripotency Sustaining Factor in Embryonic Stem Cells},
  author={I. Chambers and D. Colby and M. Robertson and Jennifer J. Nichols and S. Lee and S. Tweedie and A. Smith},
  • I. Chambers, D. Colby, +4 authors A. Smith
  • Published 2003
  • Biology, Medicine
  • Cell
  • Embryonic stem (ES) cells undergo extended proliferation while remaining poised for multilineage differentiation. A unique network of transcription factors may characterize self-renewal and simultaneously suppress differentiation. We applied expression cloning in mouse ES cells to isolate a self-renewal determinant. Nanog is a divergent homeodomain protein that directs propagation of undifferentiated ES cells. Nanog mRNA is present in pluripotent mouse and human cell lines, and absent from… CONTINUE READING

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