Functional CpG Methylation System in a Social Insect

@article{Wang2006FunctionalCM,
  title={Functional CpG Methylation System in a Social Insect},
  author={Ying Wang and Mireia Jord{\`a} and Peter L Jones and Ryszard Maleszka and Xu Ling and Hugh M. Robertson and Craig A Mizzen and Miguel A. Peinado and Gene E. Robinson},
  journal={Science},
  year={2006},
  volume={314},
  pages={645 - 647}
}
DNA methylation systems are well characterized in vertebrates, but methylation in Drosophila melanogaster and other invertebrates remains controversial. Using the recently sequenced honey bee genome, we present a bioinformatic, molecular, and biochemical characterization of a functional DNA methylation system in an insect. We report on catalytically active orthologs of the vertebrate DNA methyltransferases Dnmt1 and Dnmt3a and b, two isoforms that contain a methyl-DNA binding domain, genomic 5… 
View on AAAS
ncbi.nlm.nih.gov
328 Citations
Highly Influential Citations
17
Background Citations
117
Methods Citations
2
Results Citations
12

Topics from this paper

    328 Citations

    Citation Type

    Citation Type

    DNA methylation with a sting: an active DNA methylation system in the honeybee.
    • M. Schaefer, F. Lyko
    • Medicine, Biology
      BioEssays : news and reviews in molecular, cellular and developmental biology
    • 2007
    TLDR
    These findings establish bees as a model to analyze the function of DNA methylation systems in invertebrate organisms and might also be important to understand evolutionary aspects ofDNA methylation in higher eukaryotes.
    DNA methylation in insects: on the brink of the epigenomic era
    TLDR
    It is suggested that insects are particularly amenable to advancing the understanding of the biological functions of DNA methylation, because insects are evolutionarily diverse, display several lineage‐specific losses ofDNA methylation and possess tractable patterns of DNAmethylation in moderately sized genomes.
    Evolutionary insights into DNA methylation in insects.
    The Function of DNA Methylation Marks in Social Insects
    TLDR
    This mini review highlights current challenges and predict future breakthroughs in the field of socioepigenomics and focuses on enzymes associated with DNA methylation, the functions ofDNA methylation in caste determination, behavioral gene regulation, and the effects of DNAmethylation on learning and memory.
    Conservation and divergence of methylation patterning in plants and animals
    TLDR
    Comparisons of DNA methylation in eight diverse plant and animal genomes found that patterns of methylation are very similar in flowering plants with methylated cytosines detected in all sequence contexts, whereas CG methylation predominates in animals.
    DNA Methylation and Genome Evolution in Honeybee: Gene Length, Expression, Functional Enrichment Covary with the Evolutionary Signature of DNA Methylation
    • J. Zeng, S. Yi
    • Biology, Medicine
      Genome biology and evolution
    • 2010
    TLDR
    An intriguing covariation of length and epigenetic status of honeybee genes is reported and it is demonstrated that a subset of high-CpG genes are conspicuously longer than expected under the evolutionary relationship alone and that they are enriched in specific functional categories.
    Author ' s personal copy Evolutionary insights into DNA methylation in insects
    Epigenetic information affects gene function and plays a critical role in development. DNA methylation is one of the most widespread epigenetic marks and has been linked to developmental plasticity…
    Epigenetics in Social Insects: A New Direction for Understanding the Evolution of Castes
    TLDR
    Research on honeybees, and, when available, other social insects, on DNA methylation and queen and worker caste differences are reviewed, and a conceptual framework for the effects of methylation on caste determination in honeybees is outlined that may help guide studies of epigenetic regulation in other polyphenic taxa.
    DNA methylation and methyl-CpG binding proteins: developmental requirements and function
    TLDR
    The advances in the understanding of the function of DNA methylation, DNA methyltransferases, and methyl-CpG binding proteins in vertebrate embryonic development are reviewed.
    DNA methylation in amphioxus: from ancestral functions to new roles in vertebrates.
    TLDR
    Comparisons of genomics and analyses of conserved synteny between amphioxus and vertebrates provide robust evidence that the DNA methylation machinery of Amphioxus represents the ancestral toolkit of chordates, and that its expansion in vertebrates was originated by the two rounds of whole-genome duplication that occurred in stem vertebrates.
    ...
    1
    2
    3
    4
    5
    ...

    References

    SHOWING 1-10 OF 18 REFERENCES
    DNA methylation in insects
    TLDR
    Assessment of methylation in insects reports in four different insect species in which DNA methylation has been analysed more thoroughly indicates an apparent functional diversity that seems to argue against a strict functional conservation.
    A Dnmt2-like protein mediates DNA methylation in Drosophila
    TLDR
    It is demonstrated that Dnmt2 is both necessary and sufficient for DNA methylation in Drosophila and a novel CpT/A-specific DNA methyltransferase activity for DnMT2 proteins is suggested.
    Genomic DNA methylation: the mark and its mediators.
    Methylation of tRNAAsp by the DNA Methyltransferase Homolog Dnmt2
    TLDR
    The sequence and the structure of DNA methyltransferase-2 (DnMT2) bear close affinities to authentic DNA cytosine methyltransferases, and human DNMT2 protein restored methylation in vitro to tRNAAsp from Dnmt2-deficient strains of mouse, Arabidopsis thaliana, and Drosophila melanogaster in a manner that was dependent on preexisting patterns of modified nucleosides.
    Methylation of genomes and genes at the invertebrate-vertebrate boundary.
    TLDR
    These studies of chordate genomes suggest that the transition from fractional to global methylation occurred close to the origin of vertebrates, as amphioxus has a typically invertebrate methylation pattern whereas primitive vertebrates have patterns that are typical of vertebrate patterns.
    Eukaryotic cytosine methyltransferases.
    TLDR
    There are multiple families of DNA (cytosine-5) methyltransferases in eukaryotes, and each family appears to be controlled by different regulatory inputs.
    Mi-2 complex couples DNA methylation to chromatin remodelling and histone deacetylation
    TLDR
    The data substantiate the mechanistic link between DNA methylation, histone deacetylation and transcriptional silencing and reveal a similarity to a known methyl CpG-binding protein.
    The Drosophila MBD2/3 protein mediates interactions between the MI-2 chromatin complex and CpT/A-methylated DNA
    TLDR
    The characterized MBD2/3 null mutant allele is characterized that caused a strong dominant suppression of position-effect variegation and also resulted in a high rate of chromosome segregation defects during early embryogenesis, suggesting the existence of methylation-dependent chromatin structures in Drosophila.
    Methylation and expression of amplified esterase genes in the aphid Myzus persicae (Sulzer).
    • L. Field
    • Biology, Medicine
      The Biochemical journal
    • 2000
    TLDR
    Results suggest that the methylation of E4 has a positive role in expression, and call into question the widely held view that methylation in invertebrate genomes is confined to regions that do not contain genes and thatethylation is always associated with gene silencing.
    Sin3: a flexible regulator of global gene expression and genome stability
    TLDR
    Although most research on Sin3 has focused on its role as a corepressor, mounting evidence suggests that Sin3 can also positively regulate transcription and provide a platform to deliver multiple combinations modifications to the chromatin, using both sequence-specific and sequence-independent mechanisms.
    ...
    1
    2
    ...