Functional Coupling of the Gαolf Variant XLGαolf with the Human Adenosine A2A Receptor

  title={Functional Coupling of the G$\alpha$olf Variant XLG$\alpha$olf with the Human Adenosine A2A Receptor},
  author={Vipa Ravyn and James Robert Bostwick},
  journal={Journal of Receptors and Signal Transduction},
  pages={241 - 258}
A recently identified novel Gαolf variant, XLGαolf, is shown to functionally couple to the human adenosine A2A receptor (A2AR). In Sf9 cells expressing A2AR, β1, and γ2, co-expression of XLGαolf increased NECA-induced [35S]GTPγS binding from approximately 130% to 300% of basal levels. Pharmacological characteristics of A2AR ligands on these cells were evaluated by using [3H]ZM241385- and [35S]GTPγS- binding assays. The rank order of the equilibrium binding constants (Kd or Ki) of adenosine… 

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