Functional Comparison of Human Colonic Carcinoma Cell Lines and Primary Small Intestinal Epithelial Cells for Investigations of Intestinal Drug Permeability and First-Pass Metabolism.

@article{Yamaura2016FunctionalCO,
  title={Functional Comparison of Human Colonic Carcinoma Cell Lines and Primary Small Intestinal Epithelial Cells for Investigations of Intestinal Drug Permeability and First-Pass Metabolism.},
  author={Yoshiyuki Yamaura and Brian D Chapron and Zhican Wang and Jonathan Himmelfarb and Kenneth E. Thummel},
  journal={Drug metabolism and disposition: the biological fate of chemicals},
  year={2016},
  volume={44 3},
  pages={
          329-35
        }
}
To further the development of a model for simultaneously assessing intestinal absorption and first-pass metabolism in vitro, Caco-2, LS180, T84, and fetal human small intestinal epithelial cells (fSIECs) were cultured on permeable inserts, and the integrity of cell monolayers, CYP3A4 activity, and the inducibility of enzymes and transporters involved in intestinal drug disposition were measured. Caco-2, T84, and fSIECs all formed tight junctions, as assessed by immunofluorescence microscopy for… CONTINUE READING

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