Functional Characterization of Different ORFs Including Luciferase‐Like Monooxygenase Genes from the Mensacarcin Gene Cluster

@article{Maier2015FunctionalCO,
  title={Functional Characterization of Different ORFs Including Luciferase‐Like Monooxygenase Genes from the Mensacarcin Gene Cluster},
  author={Sarah Maier and Tanja Heitzler and Katharina Asmus and E. Br{\"o}tz and Uwe Hardter and Katharina He{\ss}elbach and T. Paululat and Andreas Bechthold},
  journal={ChemBioChem},
  year={2015},
  volume={16}
}
  • Sarah Maier, Tanja Heitzler, +5 authors Andreas Bechthold
  • Published 2015
  • Biology, Medicine
  • ChemBioChem
  • The biologically active compound mensacarcin is produced by Streptomyces bottropensis. The cosmid cos2 contains a large part of the mensacarcin biosynthesis gene cluster. Heterologous expression of this cosmid in Streptomyces albus J1074 led to the production of the intermediate didesmethylmensacarcin (DDMM). In order to gain more insights into the biosynthesis, gene inactivation experiments were carried out by λ‐Red/ET‐mediated recombination, and the deletion mutants were introduced into the… CONTINUE READING
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