Functional Association of Nmi with Stat5 and Stat1 in IL-2- and IFN γ-Mediated Signaling

Abstract

Using the coiled-coil region of Stat5b as the bait in a yeast two-hybrid screen, we identified the association of Nmi, a protein of unknown function previously reported as an N-Myc interactor. We further show that Nmi interacts with all STATs except Stat2. We evaluated two cytokine systems, IL-2 and IFNgamma, and demonstrate that Nmi augments STAT-mediated transcription in response to these cytokines. Interestingly, Nmi lacks an intrinsic transcriptional activation domain; instead, Nmi enhances the association of CBP/p300 coactivator proteins with Stat1 and Stat5, and together with CBP/p300 can augment IL-2- and IFNgamma-dependent transcription. Therefore, our data not only reveal that Nmi can potentiate STAT-dependent transcription, but also suggest that it can augment coactivator protein recruitment to at least some members of a group of sequence-specific transcription factors.

DOI: 10.1016/S0092-8674(00)80965-4

Extracted Key Phrases

5 Figures and Tables

0100200'00'02'04'06'08'10'12'14'16
Citations per Year

1,176 Citations

Semantic Scholar estimates that this publication has 1,176 citations based on the available data.

See our FAQ for additional information.

Cite this paper

@article{Zhu1999FunctionalAO, title={Functional Association of Nmi with Stat5 and Stat1 in IL-2- and IFN γ-Mediated Signaling}, author={Minghua Zhu and Susan D. John and Maria L. Berg and Warren J Leonard}, journal={Cell}, year={1999}, volume={96}, pages={121-130} }