Function of penicillin-binding protein 2 in viability and morphology of Pseudomonas aeruginosa.

@article{Legaree2007FunctionOP,
  title={Function of penicillin-binding protein 2 in viability and morphology of Pseudomonas aeruginosa.},
  author={Blaine A Legaree and Kathy Daniels and Joel T. Weadge and Darrell Cockburn and Anthony J Clarke},
  journal={The Journal of antimicrobial chemotherapy},
  year={2007},
  volume={59 3},
  pages={
          411-24
        }
}
OBJECTIVES To investigate the function of penicillin-binding protein 2 (PBP 2) in Pseudomonas aeruginosa PAO1. METHODS The growth and morphology of P. aeruginosa cultured in the absence and presence of mecillinam was assessed. The gene encoding PBP 2, pbpA, was identified in the genome of P. aeruginosa PAO1 and both its full-length and an engineered truncated form were cloned and expressed in Escherichia coli. Site-directed mutagenesis was used to confirm Ser-327 as the catalytic nucleophile… CONTINUE READING

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