Full-term pregnancies achieved with ICSI despite high levels of sperm chromatin damage.

@article{Gandini2004FulltermPA,
  title={Full-term pregnancies achieved with ICSI despite high levels of sperm chromatin damage.},
  author={Loredana Gandini and Francesco Lombardo and Donatella Paoli and F Caruso and Patrizia Eleuteri and Giorgio Leter and Rosanna Ciriminna and Franco Culasso and Franco Dondero and Andrea Lenzi and Marcello Span{\^o}},
  journal={Human reproduction},
  year={2004},
  volume={19 6},
  pages={
          1409-17
        }
}
BACKGROUND Sperm DNA integrity is essential for the accurate transmission of genetic information. The clinical significance of this assessment lies in its association with not only natural conception rates, but also the success of assisted reproduction technology (ART). It has been reported that sperm chromatin structure assay (SCSA) identified thresholds for negative pregnancy outcome after ART when the DNA fragmentation index (DFI), previously known as COMPalphat, was >30%. METHODS In a… 
Successful outcomes achieved in assisted reproduction cycles using sperm with high levels of high DNA stainability
TLDR
There were no significant statistical relationships between %HDS and fertilization rate, rate of embryo growth, blastocyst rate, implantation rate, or live birth rate, and rate of pregnancy loss showed a negative relationship significant at the 0.05 level which is unexplained.
Sperm DNA integrity assessment in prediction of assisted reproduction technology outcome.
TLDR
DFI can be used as an independent predictor of fertility in couples undergoing IUI and all infertile men should be tested with SCSA as a supplement to the standard semen analysis, when DFI exceeds 30%, ICSI should be the method of choice.
The sperm chromatin structure assay as a diagnostic tool in the human fertility clinic.
TLDR
SCSA is a useful tool in andrological diagnosis and contributes with a prognosis for the fertility outcome of conventional IVF, although full-term pregnancy can be achieved with assisted reproductive techniques with a DFI >27%, the probability of a successful pregnancy may be reduced.
The clinical significance of sperm DNA damage detection combined with routine semen testing in assisted reproduction.
TLDR
The detection of damaged DNA in spermatozoa needs to be conducted along with standard semen analysis and might prove to be a promising predictor of ART outcome, particularly in IVF.
Fall in implantation rates following ICSI with sperm with high DNA fragmentation.
TLDR
Observations may help to resolve the issues about how, and to what extent, sperm DNA damage impacts upon the success of IVF and ICSI procedures.
Clinical implications of sperm DNA damage
TLDR
The relationships between in vitro fertilisation, ICSI pregnancy rates and sperm DNA damage are reported, using the Comet assay to measure DNA fragmentation and also a novel test to measure modified bases, as a indication of oxidative DNA injury.
First recorded pregnancy and normal birth after ICSI using electrophoretically isolated spermatozoa.
TLDR
Clinical application in a couple suffering from long-term infertility associated with extensive DNA damage in the male germ line led to the first human pregnancy following such electrophoretic sperm isolation.
Sperm Chromatin and ART (IUI, IVF and ICSI) Pregnancy
TLDR
A continuous search for better markers of male fertility has led to an increased focus on sperm chromatin integrity testing in fertility workup and ART, where the comet, TUNEL, and Sperm Chromatin Structure assays are the most frequently used.
Are sperm chromatin and DNA defects relevant in the clinic?
  • A. Zini
  • Biology, Medicine
    Systems biology in reproductive medicine
  • 2011
TLDR
It is concluded that sperm DNA damage is associated with lower natural, intra-uterine insemination (IUI), and in vitro fertilization (IVF) pregnancy rates and there is clinical value in testing couples prior to assisted reproductive technologies and in those couples with recurrent miscarriages.
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