Full sensitivity of P2times2 purinoceptor to ATP revealed by changing extracellular pH

  title={Full sensitivity of P2times2 purinoceptor to ATP revealed by changing extracellular pH},
  author={B. F. King and L. Ziganshina and J. Pintor and G. Burnstock},
  journal={British Journal of Pharmacology},
  • B. F. King, L. Ziganshina, +1 author G. Burnstock
  • Published 1996
  • Biology, Medicine
  • British Journal of Pharmacology
  • A full pharmacological characterization was carried out on a recombinant ATP‐gated ion channel (P2times2 purinoceptor) expressed in Xenopus oocytes. This slowly‐desensitizing neuronal P2times2 purinoceptor, activated by ATP (EC50 = 4.6 ± 1 μm at pH 7.4; n 4), showed the agonist potency order: ATP ≥ 2‐ MeSATP = ATPγS ≥ ATPαS < < Bz‐ATP. The receptor affinity for ATP was enhanced 5–10 fold by acidifying the bathing solution (to pH 6.5) but was diminished 4–5 fold in an alkaline solution (pH 8.0… CONTINUE READING
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