Full-length transcriptome assembly from RNA-Seq data without a reference genome.

@article{Grabherr2011FulllengthTA,
  title={Full-length transcriptome assembly from RNA-Seq data without a reference genome.},
  author={Manfred G. Grabherr and Brian J. Haas and Moran Yassour and Joshua Z. Levin and Dawn A Thompson and Ido Amit and Xian Adiconis and Lin Fan and Raktima Raychowdhury and Qiandong Zeng and Zehua Chen and Evan Mauceli and Nir Hacohen and Andreas Gnirke and Nicholas Rhind and Federica Di Palma and Bruce W. Birren and Chad Nusbaum and Kerstin Lindblad-Toh and Nir Friedman and Aviv Regev},
  journal={Nature biotechnology},
  year={2011},
  volume={29 7},
  pages={
          644-52
        }
}
Massively parallel sequencing of cDNA has enabled deep and efficient probing of transcriptomes. [] Key Result By efficiently constructing and analyzing sets of de Bruijn graphs, Trinity fully reconstructs a large fraction of transcripts, including alternatively spliced isoforms and transcripts from recently duplicated genes.
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