Full-length single-stranded PCR product mediated chromosomal integration in intact Bacillus subtilis.

Abstract

The research introduced a novel method for gene replacement in intact Bacillus subtilis by employing full-length single-stranded (ss) DNA constructs and electro-transformation. 5' phosphorothioated lagging-strand targeting ssDNA construct was demonstrated to be highly recombinogenic, and the utility of the system was illustrated by introducing a… (More)
DOI: 10.1016/j.mimet.2012.11.012

Topics

Figures and Tables

Sorry, we couldn't extract any figures or tables for this paper.