Full-length single-stranded PCR product mediated chromosomal integration in intact Bacillus subtilis.


The research introduced a novel method for gene replacement in intact Bacillus subtilis by employing full-length single-stranded (ss) DNA constructs and electro-transformation. 5' phosphorothioated lagging-strand targeting ssDNA construct was demonstrated to be highly recombinogenic, and the utility of the system was illustrated by introducing a… (More)
DOI: 10.1016/j.mimet.2012.11.012


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